... Herein, we included 47 advanced non–small-cell lung cancer patients with PE, who had lung cancer driver mutations tested on tumor tissue specimens either at diagnosis or during disease progression. The supernatant and cell pellet of each PE were evaluated for molecular profiles in parallel on an Ion Torrent next-generation sequencing platform... In conclusion, our study indicates that supernatant cfTNA isolated from pleural fluids can be effectively used in clinical practice for molecular analysis by NGS. The Oncomine Lung cfTNA assay allows for detecting extremely low-abundance genomic aberrations in supernatants of pleural effusions, even in cases where corresponding cell pellets or biopsy tissues have failed for the detection. Cell-free supernatants and cell pellets could potentially complement each other and improve the detection rate of somatic genetic variants in pleural effusions. READ ARTICLE
The Journal of Molecular Diagnostics DOI:10.1016/j.jmoldx.2020.01.011
Authors: Chan Xiang, Mingfei Huo, Shengji Ma, Lianying Guo, Ruiying Zhao, Haohua Teng, Jie Zhang, Yuchen Han