Context: Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are common methods to detect ALK status in inflammatory myofibroblastic tumors (IMTs). However, equivocal ALK FISH signals and inconsistency between FISH and IHC are occasionally observed. Objective: To study the inconsistency between FISH and IHC, and clarify ALK status in IMT by targeted RNA sequencing (RNAseq). Conclusions: These findings indicated that RNAseq can simultaneously detect multiple gene fusions and provide fusion forms and breakpoints, which is of great value for differential diagnosis, especially for those uncommon IMTs with equivocal FISH findings, or inconsistency between IHC and FISH. READ ARTICLE
Archives of Pathology & Laboratory Medicine DOI:10.5858/arpa.2021-0230-OA
Authors: Qianlan Yao, Qianming Bai, Xin Zhang, Gang Ji, Heng Chang, Xu Cai, Lin Yu, Jian Wang, Xiaoli Zhu, Xiaoyan Zhou